General Information

  • ID:  hor005818
  • Uniprot ID:  P84773
  • Protein name:  bradykinin
  • Gene name:  NA
  • Organism:  Amia calva (Bowfin)
  • Family:  Bradykinin-related peptide family
  • Source:  animal
  • Expression:  Plasma.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Amia (genus), Amiidae (family), Amiiformes (order), Holostei (infraclass), Neopterygii (subclass), Actinopteri (class), Actinopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0003674 molecular_function; GO:0090729 toxin activity
  • GO BP:  GO:0035821 modulation of process of another organism; GO:0042310 vasoconstriction; GO:0042311 vasodilation; GO:0045776 negative regulation of blood pressure; GO:0045777 positive regulation of blood pressure; GO:0045986 negative regulation of smooth muscle contraction; GO:0045987 positive regulation of smooth muscle contraction; GO:0097746 blood vessel diameter maintenance
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  RPPGWSPFR
  • Length:  9
  • Propeptide:  RPPGWSPFR
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Induces smooth muscle dilation and contraction. Intra-arterial injection induces a significant decrease in ventral aortic blood pressure of between 5 and 10 minutes duration followed by a dose dependent increase in ventral aortic blood pressure of between 30 and 60 minutes duration. May target bradykinin receptors (BDKRB).
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005818_AF2.pdbhor005818_ESM.pdb

Physical Information

Mass: 124211 Formula: C52H74N16O11
Absent amino acids: ACDEHIKLMNQTVY Common amino acids: P
pI: 12.5 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 2
Hydrophobicity: -145.56 Boman Index: -2699
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 0
Instability Index: 13562.22 Extinction Coefficient cystines: 5500
Absorbance 280nm: 687.5

Literature

  • PubMed ID:  7651903
  • Title:  Isolation and biological activity of [Trp5]bradykinin from the plasma of the phylogenetically ancient fish, the bowfin and the longnosed gar.